Single-molecule mechanical unfolding of amyloidogenic beta2-microglobulin: the force-spectroscopy approach

Chemphyschem. 2009 Jul 13;10(9-10):1471-7. doi: 10.1002/cphc.200900220.

Abstract

The recombinant production of a novel chimeric polyprotein is described. The new protein contains either wild-type beta(2)-microglobulin (beta(2)m) or its truncated variant (DeltaN6 beta(2)m) (see picture). Structural characterization is achieved by means of single-molecule force spectroscopy studies of specific beta(2)m regions which could be involved in amyloidogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloidosis
  • Microscopy, Atomic Force
  • Protein Engineering
  • Protein Folding
  • Recombinant Proteins / chemistry
  • Spectrometry, Fluorescence
  • beta 2-Microglobulin / chemistry*

Substances

  • Recombinant Proteins
  • beta 2-Microglobulin