Abstract
The involvement of Ca2+ entry via the Na+/Ca2+ exchanger (NCX) in myogenic constriction of rat posterior cerebral arteries was investigated. RT-PCR identified mRNA for NCX1, 2, and 3 in the arteries. Na+ removal increased [Ca2+]i, which was reduced by the NCX inhibitor SEA0400. SEA0400 inhibited the development, but not the steady-state, of pressure-induced myogenic constriction, whereas it decreased both the initial and sustained phases of [Ca2+]i elevation. These results suggest that Ca2+ entry via NCX is involved in the development, but not the steady-state, of pressure-induced myogenic constriction.
MeSH terms
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Angiography
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Aniline Compounds / pharmacology
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Animals
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Calcium / metabolism*
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Male
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Phenyl Ethers / pharmacology
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Posterior Cerebral Artery / drug effects
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Posterior Cerebral Artery / metabolism*
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RNA, Messenger / metabolism
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Rats
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Rats, Wistar
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Reverse Transcriptase Polymerase Chain Reaction
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Sodium-Calcium Exchanger / antagonists & inhibitors
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Sodium-Calcium Exchanger / metabolism*
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Vasoconstriction / drug effects
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Vasoconstriction / physiology
Substances
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Aniline Compounds
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Phenyl Ethers
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RNA, Messenger
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SEA 0400
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Slc8a2 protein, rat
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Slc8a3 protein, rat
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Sodium-Calcium Exchanger
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sodium-calcium exchanger 1
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Calcium