Objective: To investigate the influential factors and establish culture method for G. uralensis callus.
Methods: To study the possible effective factors of culture condition by comparing with different explants, light, plant hormones and its ratio.
Results: The hypocotyl was the best among different explants, its inducing ratios was 94%, and the callus occurred earliest. When the calluses were inoculated on MS + 6 - BA (1.0-2.0) mg/L + NAA (0.5-1.0) mg/L, adventitious buds formed. 2,4-D could induce non-embryogenic callus, the compounding proportions of 6 - BA and NAA could induce embryogenic callus. Light influenced induction and growth of callus.
Conclusion: Different explants, components of hormone and light are the influencial factors of callus induction and growth of G. uralensis.