Histidine analogues of oxytocin and vasopressin as efficient ligands for Zn2+ ions--potentiometric and NMR studies

Justyna Brasuń; Marek Cebrat; Mariusz Jaremko; Łukasz Jaremko; Olimpa Gładysz; Igor Zhukov

doi:10.1016/j.jinorgbio.2009.04.016

Histidine analogues of oxytocin and vasopressin as efficient ligands for Zn2+ ions--potentiometric and NMR studies

J Inorg Biochem. 2009 Jul;103(7):1033-8. doi: 10.1016/j.jinorgbio.2009.04.016. Epub 2009 May 10.

Authors

Justyna Brasuń¹, Marek Cebrat, Mariusz Jaremko, Łukasz Jaremko, Olimpa Gładysz, Igor Zhukov

Affiliation

¹ Department of Inorganic Chemistry, Wrocław Medical University, Wrocław, Poland. [email protected]

PMID: 19515425
DOI: 10.1016/j.jinorgbio.2009.04.016

Abstract

We have characterized the interaction between the Zn(2+) ions and the histidine analogues of oxytocin and arginine-vasopressin. Potentiometric methods were used for the determination of the stoichiometry of the complexes formed and the calculation of their stability constants. The NMR measurements revealed detailed structures of the complexes and confirmed the binding mode at physiological pH.

MeSH terms

Arginine Vasopressin / analogs & derivatives*
Cations, Divalent / chemistry
Histidine / chemistry*
Ligands
Magnetic Resonance Spectroscopy
Nuclear Magnetic Resonance, Biomolecular
Oxytocin / analogs & derivatives*
Potentiometry
Zinc / chemistry*

Substances

Cations, Divalent
Ligands
Arginine Vasopressin
Histidine
Oxytocin
Zinc