Abstract
Human and simian immunodeficiency viruses (HIV/SIV) exhibit enormous sequence heterogeneity within each infected host. Here, we use ultradeep pyrosequencing to create a comprehensive picture of CD8(+) T-lymphocyte (CD8-TL) escape in SIV-infected macaques, revealing a previously undetected complex pattern of viral variants. This increased sensitivity enabled the detection of acute CD8-TL escape as early as 17 days postinfection, representing the earliest published example of CD8-TL escape in intrarectally infected macaques. These data demonstrate that pyrosequencing can be used to study the evolution of CD8-TL escape during immunodeficiency virus infection with an unprecedented degree of sensitivity.
Publication types
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Evaluation Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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CD8-Positive T-Lymphocytes / chemistry
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CD8-Positive T-Lymphocytes / immunology
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CD8-Positive T-Lymphocytes / virology*
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Epitopes / chemistry
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Epitopes / genetics
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Epitopes / immunology
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Genetic Variation
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Macaca mulatta
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Molecular Sequence Data
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Sequence Alignment
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Sequence Analysis, DNA / methods*
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Simian Acquired Immunodeficiency Syndrome / genetics
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Simian Acquired Immunodeficiency Syndrome / immunology
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Simian Acquired Immunodeficiency Syndrome / virology*
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Simian Immunodeficiency Virus / chemistry
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Simian Immunodeficiency Virus / genetics*
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Simian Immunodeficiency Virus / immunology
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Viral Proteins / chemistry
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Viral Proteins / genetics