Abstract
We describe a new technology for the molecular genotyping of microbes using a platform known commercially as the Ibis T5000. The technology couples multilocus polymerase chain reaction (PCR) to electrospray ionization/mass spectrometry (PCR/ESI-MS) and was developed to provide rapid, high-throughput, and precise digital analysis of either isolated colonies or original patient specimens on a platform suitable for use in hospital or reference diagnostic laboratories or public health settings. The PCR/ESI-MS method measures digital molecular signatures from microbes, enabling real-time epidemiological surveillance and outbreak investigation. This technology will facilitate understanding of the pathways by which infectious organisms spread and will enable appropriate interventions on a time frame not previously achievable.
MeSH terms
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Acinetobacter Infections / epidemiology
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Acinetobacter Infections / microbiology
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Acinetobacter baumannii / genetics
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Acinetobacter baumannii / isolation & purification
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Cross Infection / diagnosis
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Cross Infection / microbiology
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Cross Infection / prevention & control*
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DNA, Bacterial / genetics
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DNA, Bacterial / isolation & purification
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DNA, Viral / genetics
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DNA, Viral / isolation & purification
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Disease Outbreaks
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Genes, Bacterial
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Genetics, Microbial / methods*
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Genotype
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Humans
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Influenza A virus / genetics
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Influenza A virus / isolation & purification
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Molecular Epidemiology / methods*
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Polymerase Chain Reaction / methods
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Polymorphism, Single Nucleotide
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Population Surveillance / methods*
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Spectrometry, Mass, Electrospray Ionization / methods
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Streptococcal Infections / epidemiology
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Streptococcal Infections / microbiology
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Streptococcus pyogenes / genetics
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Streptococcus pyogenes / isolation & purification
Substances
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DNA, Bacterial
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DNA, Viral