Abstract
We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Agouti Signaling Protein / genetics*
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Animals
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Base Sequence
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Cell Line
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Crosses, Genetic
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DNA Primers / genetics
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Embryonic Stem Cells / cytology*
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Embryonic Stem Cells / metabolism*
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Female
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Gene Targeting
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Genetic Techniques
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Germ-Line Mutation
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Hair Color / genetics
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Male
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Mice
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Mice, Inbred C57BL / classification
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Mice, Inbred C57BL / genetics*
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Mice, Knockout
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Mice, Mutant Strains
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Mice, Transgenic
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Polymorphism, Single Nucleotide
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Pregnancy
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Transplantation Chimera / genetics
Substances
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Agouti Signaling Protein
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DNA Primers
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a protein, mouse