Abstract
The family of leptospiral immunoglobulin-like (lig) genes comprises ligA, ligB and ligC. This study used PCR to demonstrate the presence of lig genes among serovars from a collection of leptospiral strains and clinical isolates. Whilst ligA and ligC appeared to be present in a limited number of pathogenic serovars, the ligB gene was distributed ubiquitously among all pathogenic strains. None of the lig genes were detected among intermediate or saprophytic Leptospira species. It was also shown that, similar to the previously characterized secY gene, a short specific PCR fragment of ligB could be used to correctly identify pathogenic Leptospira species. These findings demonstrate that ligB is widely present among pathogenic strains and may be useful for their reliable identification and classification.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Bacterial / chemistry
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Antigens, Bacterial / genetics*
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Antigens, Bacterial / immunology
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Bacterial Typing Techniques
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Base Sequence
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DNA, Bacterial / genetics
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Gene Expression Regulation, Bacterial / physiology
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Leptospira / classification*
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Leptospira / genetics*
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Leptospira / immunology
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Leptospira / pathogenicity
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Molecular Sequence Data
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Phylogeny
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RNA, Bacterial / genetics
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RNA, Ribosomal, 16S / genetics
Substances
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Antigens, Bacterial
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DNA, Bacterial
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LigB-m protein, Leptospira interrogans
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RNA, Bacterial
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RNA, Ribosomal, 16S
Associated data
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GENBANK/EU938447
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GENBANK/EU938448
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GENBANK/EU938449
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GENBANK/EU938450
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GENBANK/EU938451
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GENBANK/EU938452
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GENBANK/EU938454
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GENBANK/EU938455
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GENBANK/EU938456
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GENBANK/EU938457
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GENBANK/EU938459
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GENBANK/EU938460
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GENBANK/EU938461
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