Abstract
Paramagnetic effects offer a rich source of long-range structural restraints. Here we review current methods for site-specific tagging of proteins and oligonucleotides with paramagnetic molecules. The paramagnetic tags include nitroxide radicals and metal chelators. Particular emphasis is placed on tags suitable for site-specific and rigid attachment of lanthanide ions to macromolecules.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Electron Spin Resonance Spectroscopy / methods*
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Lanthanoid Series Elements / chemistry
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Models, Molecular
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Nuclear Magnetic Resonance, Biomolecular / methods*
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Oligonucleotides / chemistry*
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Protein Conformation*
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Proteins / chemistry*
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Spin Labels
Substances
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Lanthanoid Series Elements
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Oligonucleotides
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Proteins
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Spin Labels