Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis

Chem Commun (Camb). 2009 May 14:(18):2484-6. doi: 10.1039/b902240f. Epub 2009 Apr 6.

Abstract

The Michaelis complex of the beta-mannosidase Man2A shows distortion to a (1)S(5) conformation adding to the growing body of evidence supporting catalysis through a boat conformation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteroides / enzymology
  • Hydrolysis
  • Mannosides / chemistry*
  • Mannosides / metabolism
  • Models, Molecular
  • Molecular Conformation*
  • Thermodynamics
  • beta-Mannosidase / chemistry*
  • beta-Mannosidase / metabolism

Substances

  • Mannosides
  • beta-Mannosidase