Abstract
Faithful chromatin segregation is mediated and controlled by the kinetochore protein network which assembles at centromeres. In this study, the neighbourhood relations of inner kinetochore and nucleosome-associated complex (NAC) proteins were analysed in living human interphase cells by acceptor photobleaching FRET. The data indicate that CENP-U is in close vicinity to CENP-I as well as to CENP-B and that CENP-M is close to CENP-T.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Blotting, Western
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Cell Line, Tumor
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Centromere Protein B / chemistry
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Centromere Protein B / metabolism
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Chromosomal Proteins, Non-Histone / chemistry
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Chromosomal Proteins, Non-Histone / metabolism
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Fluorescence Resonance Energy Transfer / methods
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Green Fluorescent Proteins / genetics
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Humans
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Interphase / physiology
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Kinetochores / chemistry*
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Kinetochores / metabolism
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Luminescent Proteins / genetics
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Nuclear Proteins / chemistry*
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Nuclear Proteins / metabolism
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Photobleaching
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Transfection
Substances
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Bacterial Proteins
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CENPB protein, human
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CENPT protein, human
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Centromere Protein B
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Chromosomal Proteins, Non-Histone
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Luminescent Proteins
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Nuclear Proteins
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centromere protein C
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enhanced green fluorescent protein
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yellow fluorescent protein, Bacteria
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Green Fluorescent Proteins