Abstract
Glycolipids are presented to T cells by human group 1 CD1 proteins, but are not used as subunit vaccines yet. Experimental immunizations with pure mycobacterial glucose monomycolate (GMM) and keyhole limpet haemocyanin (KLH) in cattle, a species which, unlike mice, expresses group 1 CD1, showed that GMM was equally efficient as KLH in generating T cell responses in blood, but not in the draining lymph node. Also, KLH induced strong antibody responses whereas GMM did not. These data suggest that non-overlapping T cell populations are targeted and demonstrate the potential of glycolipids as a special class of subunit vaccine candidates.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / administration & dosage
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Adjuvants, Immunologic / pharmacology
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Animals
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Antibodies, Bacterial / immunology
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Cattle
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Glycolipids / immunology*
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Glycolipids / isolation & purification
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Hemocyanins / immunology
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Immunologic Memory*
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Mycobacterium bovis / chemistry*
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Mycobacterium bovis / immunology
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Quaternary Ammonium Compounds / administration & dosage
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Quaternary Ammonium Compounds / pharmacology
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T-Lymphocytes / immunology*
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Tuberculosis Vaccines / immunology*
Substances
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Adjuvants, Immunologic
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Antibodies, Bacterial
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Glycolipids
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Quaternary Ammonium Compounds
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Tuberculosis Vaccines
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glucose mycolate
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dimethyldioctadecylammonium
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Hemocyanins
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keyhole-limpet hemocyanin