Abstract
Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cytomegalovirus / genetics
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Fibroblasts / virology
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Genes, Essential*
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Genes, Viral*
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Genetic Complementation Test
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Genome, Viral
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Herpesviridae / drug effects
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Herpesviridae / genetics*
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Herpesviridae / metabolism
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Humans
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Ligands
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Mice
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Morpholines / pharmacology
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Mutation
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Protein Stability
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Recombinant Fusion Proteins / genetics
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Viral Proteins / genetics*
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Virus Replication / drug effects
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Virus Replication / genetics
Substances
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Ligands
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Morpholines
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Recombinant Fusion Proteins
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Shield-1 compound
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Viral Proteins