Abstract
Proteases produced by immune cells have been found to be important components of the immune response to antigen. A protease previously unrecognized as a specific T cell product has been identified which has the gene sequence, serologic crossreactivity, and enzymatic specificity of elastase. T cell elastase, found in combination with the natural elastase inhibitor alpha 1-antitrypsin (alpha 1-protease inhibitor, alpha 1-PI), is produced by both CD4+ and CD8+ T lymphocytes, and is found both in a membrane-bound and in a soluble form in murine T cell lines.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal
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Base Sequence
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Blotting, Western
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CD4 Antigens / analysis
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CD8 Antigens / analysis
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Enzyme-Linked Immunosorbent Assay
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Hybridomas / enzymology
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Hybridomas / immunology
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Kinetics
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Mice
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Pancreas / enzymology
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Pancreatic Elastase / genetics
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Pancreatic Elastase / isolation & purification
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Pancreatic Elastase / metabolism*
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Polymerase Chain Reaction
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Substrate Specificity
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Swine
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T-Lymphocyte Subsets / enzymology
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T-Lymphocyte Subsets / immunology
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T-Lymphocytes / enzymology*
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T-Lymphocytes / immunology
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alpha 1-Antitrypsin / isolation & purification
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alpha 1-Antitrypsin / metabolism
Substances
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Antibodies, Monoclonal
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CD4 Antigens
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CD8 Antigens
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Oligodeoxyribonucleotides
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alpha 1-Antitrypsin
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Pancreatic Elastase