Ligation or cross-linking of CD40 has different effects on AGS gastric cancer cells

Chun-Jian Qi; Ke-Qing Qian; Yong-Ling Ning; Hong-Bing Ma; Shi-Zhong Wang; Xue-Guang Zhang

doi:10.1016/j.cellimm.2009.06.007

Ligation or cross-linking of CD40 has different effects on AGS gastric cancer cells

Cell Immunol. 2009;259(2):135-40. doi: 10.1016/j.cellimm.2009.06.007. Epub 2009 Jun 21.

Authors

Chun-Jian Qi¹, Ke-Qing Qian, Yong-Ling Ning, Hong-Bing Ma, Shi-Zhong Wang, Xue-Guang Zhang

Affiliation

¹ Department of Oncology, the Affiliated Hospital of Nanjing Medical University, Changzhou Second People's Hospital, Changzhou, PR China.

PMID: 19604505
DOI: 10.1016/j.cellimm.2009.06.007

Abstract

A gastric cancer (GC) cell line, AGS, has high-level expression of CD40, a tumor necrosis factor receptor (TNFR) family member. CD40 is present on the surfaces of a large variety of cells, including B cells, endothelial cells, dendritic cells and some carcinoma cells, and delivers signals regulating diverse cellular responses, such as proliferation, differentiation, growth suppression, and cell death. In this research, we studied the effects of different forms of CD40 stimulation on AGS cells by flow cytometry, Western blotting and siRNA transfection. We found that different forms of CD40 stimulation, either recombinant soluble CD40L (sCD40L, ligation) or agonist anti-CD40 antibody (cross-linking), induced different effects in AGS gastric cancer cells, proliferation or apoptosis. We also showed that VEGF provided a significant contribution to sCD40L-induced proliferation, while agonist anti-CD40 antibody induced GADD45 upregulation and promoted apoptosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis / immunology
CD40 Antigens / immunology*
CD40 Antigens / pharmacology
CD40 Ligand / immunology*
CD40 Ligand / pharmacology
Cell Cycle Proteins / genetics
Cell Cycle Proteins / immunology
Cell Line, Tumor
Cell Proliferation
Chromones / pharmacology
Flow Cytometry
Humans
Indoles / pharmacology
Morpholines / pharmacology
Nuclear Proteins / genetics
Nuclear Proteins / immunology
Protein Kinase Inhibitors / pharmacology
Pyrroles / pharmacology
RNA / chemistry
RNA / genetics
RNA, Small Interfering / genetics
Recombinant Proteins / pharmacology
Reverse Transcriptase Polymerase Chain Reaction
Stomach Neoplasms / immunology*
Vascular Endothelial Growth Factor A / immunology

Substances

CD40 Antigens
Cell Cycle Proteins
Chromones
GADD45A protein, human
Indoles
Morpholines
Nuclear Proteins
Protein Kinase Inhibitors
Pyrroles
RNA, Small Interfering
Recombinant Proteins
Vascular Endothelial Growth Factor A
CD40 Ligand
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
RNA
Semaxinib