Hepatitis B virus and hepatitis delta virus genotypes in outbreaks of fulminant hepatitis (Labrea black fever) in the western Brazilian Amazon region

M S Gomes-Gouvêa; M C P Soares; G Bensabath; I M V G de Carvalho-Mello; E M F Brito; O S C Souza; A T L Queiroz; F J Carrilho; J R R Pinho

doi:10.1099/vir.0.013615-0

Hepatitis B virus and hepatitis delta virus genotypes in outbreaks of fulminant hepatitis (Labrea black fever) in the western Brazilian Amazon region

J Gen Virol. 2009 Nov;90(Pt 11):2638-2643. doi: 10.1099/vir.0.013615-0. Epub 2009 Jul 15.

Authors

M S Gomes-Gouvêa^{1

2}, M C P Soares¹, G Bensabath¹, I M V G de Carvalho-Mello^{3

2}, E M F Brito¹, O S C Souza¹, A T L Queiroz⁴, F J Carrilho², J R R Pinho²

Affiliations

¹ Seção de Hepatologia, Instituto Evandro Chagas, Avenida Almirante Barroso 492, Belém, PA 66090-000, Brazil.
² Laboratory of Tropical Hepatology and Gastroenterology, Tropical Medicine Institute, Department of Gastroenterology, University of São Paulo Medical School, Av. Dr Enéas Carvalho de Aguiar 500, São Paulo, SP 05403-000, Brazil.
³ Laboratório de Imunologia Viral, Instituto Butantan, Avenida Vital Brasil 1500, São Paulo, SP 05503-900, Brazil.
⁴ Instituto de Biociências, Universidade de São Paulo, Rua do Matão 277, São Paulo, SP 05508-900, Brazil.

PMID: 19605587
DOI: 10.1099/vir.0.013615-0

Abstract

The genotypes of hepatitis B (HBV) and delta (HDV) viruses circulating among fulminant hepatitis cases from the western Amazon Basin of Brazil were characterized in this study. HBV and HDV isolates were obtained from liver samples from 14 patients who developed fulminant hepatitis and died during 1978-1989. HBV DNA and HDV RNA were detected in all samples. Phylogenetic analyses of HDV sequences showed that they all clustered with previously characterized sequences of HDV genotype 3 (HDV-3). HBV genotypes F, A and D were found in 50.0, 28.6 and 21.4 % of cases, respectively. These results confirm the predominance of HDV-3 in South America and its association with the severe form of hepatitis, and the finding of the co-infection of HDV-3 with different genotypes of HBV suggests that the association between HDV-3 and HBV-F is not necessarily causally related to a more severe clinical course of infection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Brazil / epidemiology
Cluster Analysis
DNA, Viral / genetics
Disease Outbreaks*
Genotype
Hepatitis B / epidemiology*
Hepatitis B / virology
Hepatitis B virus / classification*
Hepatitis B virus / genetics
Hepatitis B virus / isolation & purification
Hepatitis D / epidemiology*
Hepatitis D / virology
Hepatitis Delta Virus / classification*
Hepatitis Delta Virus / genetics
Hepatitis Delta Virus / isolation & purification
Humans
Liver / virology
Molecular Sequence Data
Phylogeny
RNA, Viral / genetics
Sequence Analysis, DNA

Substances

DNA, Viral
RNA, Viral

Associated data

GENBANK/FJ010634
GENBANK/FJ010635
GENBANK/FJ010636
GENBANK/FJ010637
GENBANK/FJ010638
GENBANK/FJ010639
GENBANK/FJ010640
GENBANK/FJ010641
GENBANK/FJ010642
GENBANK/FJ010643
GENBANK/FJ010644
GENBANK/FJ010645
GENBANK/FJ010646
GENBANK/FJ010647
GENBANK/FJ010648
GENBANK/FJ010649
GENBANK/FJ010650
GENBANK/FJ010651
GENBANK/FJ010652
GENBANK/FJ010653
GENBANK/FJ010654
GENBANK/FJ010655
GENBANK/FJ010656
GENBANK/FJ010657
GENBANK/FJ010658
GENBANK/FJ010659
GENBANK/FJ010660
GENBANK/FJ010661