Abstract
Histone acetylation and deacetylation are important for gene regulation. The histone acetyltransferase, Gcn5, is an activator of transcriptional initiation that is recruited to gene promoters. Here, we map genome-wide Gcn5 occupancy and histone H3K14ac at high resolution. Gcn5 is predominantly localized to coding regions of highly transcribed genes, where it collaborates antagonistically with the class-II histone deacetylase, Clr3, to modulate H3K14ac levels and transcriptional elongation. An interplay between Gcn5 and Clr3 is crucial for the regulation of many stress-response genes. Our findings suggest a new role for Gcn5 during transcriptional elongation, in addition to its known role in transcriptional initiation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylation
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Acetyltransferases / genetics
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Acetyltransferases / metabolism*
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism*
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Gene Expression Regulation, Fungal
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Genome, Fungal
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Histone Acetyltransferases / genetics
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Histone Acetyltransferases / metabolism*
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Histone Deacetylases / metabolism*
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Histones
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Open Reading Frames*
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Schizosaccharomyces pombe Proteins / genetics
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Schizosaccharomyces pombe Proteins / metabolism*
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Stress, Physiological*
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Transcription, Genetic*
Substances
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Cell Cycle Proteins
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Clr3 protein, S pombe
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Histones
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Schizosaccharomyces pombe Proteins
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Acetyltransferases
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Gcn5 protein, S pombe
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Histone Acetyltransferases
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Histone Deacetylases