1H, 13C, and 15N NMR assignments of an engineered intein based on Mycobacterium tuberculosis RecA

Biomol NMR Assign. 2008 Dec;2(2):111-3. doi: 10.1007/s12104-008-9098-4. Epub 2008 May 30.

Abstract

The backbone and side chain resonance assignments of an engineered intein based on Mycobacterium tuberculosis RecA have been determined based on triple-resonance experiments with the uniformly [(13)C,(15)N]-labeled protein.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Carbon Isotopes / chemistry
  • Inteins*
  • Magnetic Resonance Spectroscopy / methods*
  • Molecular Sequence Data
  • Molecular Weight
  • Mycobacterium tuberculosis / metabolism*
  • Nitrogen Isotopes / chemistry
  • Protein Engineering / methods*
  • Protein Structure, Tertiary
  • Protons
  • Rec A Recombinases / chemistry*

Substances

  • Carbon Isotopes
  • Nitrogen Isotopes
  • Protons
  • Rec A Recombinases