Abstract
Helicobacter pylori, a microaerophilic gram-negative bacterium, colonizes the human stomach. About 50% of the world's population is infected, and this infection is considered as the major risk factor for the development of gastric adenocarcinomas in 1% of infected subjects. Carcinogenesis is characterized by the process of epithelial-to-mesenchymal transition (EMT), in the course of which fully differentiated epithelial cells turn into depolarized and migratory cells. Concomitant disruption of adherence junctions (AJ) is facilitated by growth factors like hepatocyte growth factor 1 (HGF-1), but has been also shown to depend on ectodomain shedding of E-cadherin. The aim of this study was to investigate the impact of infection with H. pylori of NCI-N87 gastric epithelial cells on the shedding of E-cadherin and HGF-receptor c-Met. Our results show that infection with H. pylori provokes shedding of the surface proteins c-Met and E-cadherin. Evidence is provided that ADAM10 contributes to the shedding of c-Met and E-cadherin.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
ADAM Proteins / genetics
-
ADAM Proteins / metabolism
-
ADAM10 Protein
-
Amyloid Precursor Protein Secretases / genetics
-
Amyloid Precursor Protein Secretases / metabolism
-
Cadherins / metabolism*
-
Cell Line
-
Disease Progression
-
Epithelial Cells / metabolism*
-
Epithelial Cells / microbiology*
-
Gene Expression / genetics
-
Glycine / analogs & derivatives
-
Glycine / pharmacology
-
Helicobacter Infections / metabolism*
-
Helicobacter Infections / microbiology
-
Helicobacter pylori / physiology*
-
Humans
-
Hydroxamic Acids / pharmacology
-
Membrane Proteins / genetics
-
Membrane Proteins / metabolism
-
Metalloendopeptidases / antagonists & inhibitors
-
Metalloendopeptidases / metabolism
-
Peptide Fragments / metabolism*
-
Protease Inhibitors / pharmacology
-
Proto-Oncogene Proteins c-met / metabolism*
-
RNA, Small Interfering / genetics
-
Stomach / cytology
-
Stomach Neoplasms / metabolism
-
Stomach Neoplasms / microbiology
-
Stomach Neoplasms / pathology
-
Transfection
Substances
-
Cadherins
-
Hydroxamic Acids
-
KB R7785
-
Membrane Proteins
-
Peptide Fragments
-
Protease Inhibitors
-
RNA, Small Interfering
-
TAPI-2
-
Proto-Oncogene Proteins c-met
-
Amyloid Precursor Protein Secretases
-
ADAM Proteins
-
ADAM19 protein, human
-
Metalloendopeptidases
-
ADAM10 Protein
-
ADAM10 protein, human
-
Glycine