Abstract
We designed and tested three PilA-derived vaccine candidates in a chinchilla model of ascending nontypeable Haemophilus influenzae (NTHI)-induced otitis media (OM). Delivery of antiserum directed against each immunogen conferred varying degrees of protection. Presentation of a B-cell epitope derived from the OMP P5 adhesin at the N-terminus of recombinant soluble PilA protein (as opposed to the C-terminus), resulted in a protective chimeric immunogen that combined epitopes from two distinct NTHI adhesins (type IV pili and OMP P5). Incorporating protective epitopes derived from two NTHI adhesins/virulence determinants into a single pediatric vaccine candidate to prevent OM has multiple potential inherent advantages.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Adhesins, Bacterial / immunology*
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Adult
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Amino Acid Sequence
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Animals
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Antibodies, Bacterial / blood
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Antibodies, Bacterial / immunology
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Bacterial Vaccines / immunology*
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Biosensing Techniques
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Child, Preschool
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Chinchilla
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Epitope Mapping*
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Epitopes, B-Lymphocyte / immunology*
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Haemophilus Infections / immunology
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Haemophilus Infections / prevention & control*
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Haemophilus influenzae / immunology
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Humans
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Immunodominant Epitopes / immunology
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Infant
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Models, Molecular
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Molecular Sequence Data
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Otitis Media with Effusion / immunology
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Otitis Media with Effusion / prevention & control*
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Protein Structure, Tertiary
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Pulmonary Disease, Chronic Obstructive / immunology
Substances
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Adhesins, Bacterial
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Antibodies, Bacterial
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Bacterial Vaccines
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Epitopes, B-Lymphocyte
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Immunodominant Epitopes