Abstract
A mutant of Escherichia coli that employs a glycerol:nicotinamide adenine dinucleotide 2-oxidoreductase (EC 1.1.1.6), instead of adenosine 5'-triphosphate:glycerol 3-phosphotransferase (EC 2.7.1.30), as the first enzyme for the dissimilation of glycerol was constructed. This mutant, like the wild-type strain, still cannot grow anaerobically on glycerol without an exogenous hydrogen acceptor.
Publication types
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Aerobiosis
-
Alcohol Oxidoreductases / metabolism*
-
Escherichia coli / enzymology
-
Escherichia coli / growth & development
-
Escherichia coli / metabolism*
-
Glycerol / metabolism*
-
Glycerol Kinase / metabolism*
-
Mutation*
-
NAD / metabolism
-
Phosphotransferases / metabolism*
Substances
-
NAD
-
Alcohol Oxidoreductases
-
Phosphotransferases
-
Glycerol Kinase
-
Glycerol