Isolation and identification of a novel human metabolite of cyclosporin A: dihydro-CsA M17

G P Meier; S B Park; G C Yee; D J Gmur

Isolation and identification of a novel human metabolite of cyclosporin A: dihydro-CsA M17

Drug Metab Dispos. 1990 Jan-Feb;18(1):68-71.

Authors

G P Meier¹, S B Park, G C Yee, D J Gmur

Affiliation

¹ Department of Medicinal Chemistry, School of Pharmacy, University of Washington, Seattle 98195.

PMID: 1970780

Abstract

A novel metabolite of cyclosporin A was observed in human blood and urine. An analytical sample of this metabolite was isolated from human urine and the structure was determined to be (8-hydroxy-6,7-dihydro-MeBMT1) cyclosporin based on the 1H-NMR, 13C-NMR, FAB-MS, and HPLC characteristics of the biological sample as well as by comparison with a synthetically derived authentic sample. The significance of this metabolite in terms of the pathway by which cyclosporin A is metabolized is discussed.

MeSH terms

Chromatography, High Pressure Liquid
Cyclosporins / metabolism*
Cyclosporins / urine
Digestive System / metabolism
Digestive System / microbiology
Humans
Hydroxylation
Magnetic Resonance Spectroscopy
Mass Spectrometry

Substances

Cyclosporins
8-hydroxy-6,7-dihydro-(4-(2-butenyl)-4,N-dimethylthreonine)cyclosporin
dihydrocyclosporin A