OPLA scaffold, collagen I, and horse serum induce an higher degree of myogenic differentiation of adult rat cardiac stem cells

Valentina Di Felice; Nella Maria Ardizzone; Angela De Luca; Vito Marcianò; Antonella Marino Gammazza; Filippo Macaluso; Lucrezia Manente; Francesco Cappello; Antonio De Luca; Giovanni Zummo

doi:10.1002/jcp.21912

OPLA scaffold, collagen I, and horse serum induce an higher degree of myogenic differentiation of adult rat cardiac stem cells

J Cell Physiol. 2009 Dec;221(3):729-39. doi: 10.1002/jcp.21912.

Authors

Valentina Di Felice¹, Nella Maria Ardizzone, Angela De Luca, Vito Marcianò, Antonella Marino Gammazza, Filippo Macaluso, Lucrezia Manente, Francesco Cappello, Antonio De Luca, Giovanni Zummo

Affiliation

¹ Department of Experimental Medicine, Human Anatomy Section, University of Palermo, Palermo, Italy. [email protected]

PMID: 19725057
DOI: 10.1002/jcp.21912

Abstract

In the last few years, a major goal of cardiac research has been to drive stem cell differentiation to replace damaged myocardium. Several research groups have attempted to differentiate potential cardiac stem cells (CSCs) using bi- or three-dimensional systems supplemented with growth factors or molecules acting as differentiating substances. We hypothesize that these systems failed to induce a complete differentiation because they lacked an architectural space. In the present study, we isolated a pool of small proliferating and fibroblast-like cells from adult rat myocardium. The phenotype of these cells was assessed and the characterized cells were cultured in a collagen I/OPLA scaffold with horse serum to obtain fine myocardial differentiation. C-Kit(POS)/Sca-1(POS) CSCs fully differentiated in vitro when an environment more similar to the CSC niche was created. These experiments demonstrated an important model for the study of the biology of CSCs and the biochemical pathways that lead to myocardial differentiation. The results pave the way for a new surgical approach.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actins / metabolism
Adult Stem Cells / cytology*
Adult Stem Cells / metabolism
Animals
Cell Differentiation / drug effects*
Cell Separation / methods
Clone Cells / cytology
Clone Cells / metabolism
Collagen Type I / pharmacology*
Connexin 43 / metabolism
Female
GATA4 Transcription Factor / metabolism
Homeodomain Proteins / metabolism
Horses
Intermediate Filament Proteins / metabolism
LIM-Homeodomain Proteins
Microscopy, Electron, Transmission
Muscle Development / drug effects
Myocardium / cytology
Myocytes, Cardiac / cytology*
Myocytes, Cardiac / metabolism
Myocytes, Cardiac / ultrastructure
Myosin Heavy Chains / metabolism
Nerve Tissue Proteins / metabolism
Nestin
Proto-Oncogene Proteins c-kit / metabolism
Rats
Rats, Sprague-Dawley
Serum*
Tissue Scaffolds*
Transcription Factors
Troponin T / metabolism

Substances

Actins
Collagen Type I
Connexin 43
GATA4 Transcription Factor
Homeodomain Proteins
Intermediate Filament Proteins
LIM-Homeodomain Proteins
NES protein, human
Nerve Tissue Proteins
Nes protein, rat
Nestin
Transcription Factors
Troponin T
insulin gene enhancer binding protein Isl-1
smooth muscle actin, rat
Proto-Oncogene Proteins c-kit
Myosin Heavy Chains