Abstract
Hepatocellular carcinoma (HCC) is one of the world's top five causes of cancer-related deaths and treatment options are limited in this type of cancer. In the present study, we attempt to identify the novel suppressor genes of HCC using a double-combination array we designed. Leukemia inhibitory factor receptor (LIFR) is one of suppressor genes using this method. We found that 23 of 48 (47.9%) tumor tissues showed promoter hypermethylation of LIFR gene, and the expression level was clearly reduced in tumor tissues (P<0.0001). The present study suggests that our method is a meaning technique able to detect novel genes and that LIFR gene is a new suppressor gene of HCC.
Copyright 2009 Elsevier Ireland Ltd. All rights reserved.
MeSH terms
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Adult
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Aged
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Biomarkers, Tumor / genetics
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Carcinoma, Hepatocellular / genetics*
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Carcinoma, Hepatocellular / metabolism
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Carcinoma, Hepatocellular / pathology
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DNA Methylation*
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Female
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Gene Expression Profiling*
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Gene Expression Regulation, Neoplastic
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Gene Silencing
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Genes, Tumor Suppressor*
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Humans
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Leukemia Inhibitory Factor Receptor alpha Subunit / genetics*
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Leukemia Inhibitory Factor Receptor alpha Subunit / metabolism
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Liver Neoplasms / genetics*
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Liver Neoplasms / metabolism
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Liver Neoplasms / pathology
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Male
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Middle Aged
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Oligonucleotide Array Sequence Analysis*
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Polymorphism, Single Nucleotide
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Promoter Regions, Genetic / genetics
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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Biomarkers, Tumor
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LIFR protein, human
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Leukemia Inhibitory Factor Receptor alpha Subunit
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RNA, Messenger