Identification of beta 2-adrenoceptors on guinea pig alveolar macrophages using (-)-3-[125I]iodocyanopindolol

R Leurs; F D Beusenberg; A Bast; J G Van Amsterdam; H Timmerman

doi:10.1007/BF00914093

Identification of beta 2-adrenoceptors on guinea pig alveolar macrophages using (-)-3-[125I]iodocyanopindolol

Inflammation. 1990 Aug;14(4):421-6. doi: 10.1007/BF00914093.

Authors

R Leurs¹, F D Beusenberg, A Bast, J G Van Amsterdam, H Timmerman

Affiliation

¹ Department of Pharmacochemistry, Faculty of Chemistry, Vrije Universiteit, Amsterdam, The Netherlands.

PMID: 1974244
DOI: 10.1007/BF00914093

Abstract

The beta-adrenoceptor antagonist (-)-3-[125I]iodocyanopindolol ([125I]ICYP) binds with high affinity and in saturable way to membranes of guinea pig alveolar macrophages. The equilibrium dissociation constant for [125I]ICYP is 24.3 +/- 1.2 pM, and the number of binding sites is 166.3 +/- 13.7 fmol/mg protein (N = 4, +/- SEM). Displacement studies with selective antagonists showed that [125I]ICYP labels beta 2-adrenoceptors on guinea pig alveolar macrophages.

MeSH terms

Adrenergic beta-Antagonists / metabolism
Animals
Binding, Competitive
Cell Membrane / ultrastructure
Guinea Pigs
Iodine Radioisotopes
Iodocyanopindolol
Macrophages / ultrastructure*
Male
Pindolol / analogs & derivatives
Pindolol / metabolism
Propanolamines / metabolism
Pulmonary Alveoli / cytology
Receptors, Adrenergic, beta / classification
Receptors, Adrenergic, beta / metabolism*
Timolol / metabolism

Substances

Adrenergic beta-Antagonists
Iodine Radioisotopes
Propanolamines
Receptors, Adrenergic, beta
ICI 118551
Timolol
Iodocyanopindolol
Pindolol
ICI 89406