Synthesis and properties of terminally modified oligonucleotides capable of short-RNA selective hybridization

Nucleic Acids Symp Ser (Oxf). 2009:(53):13-4. doi: 10.1093/nass/nrp007.

Abstract

We have developed 2'-O-methyl-RNAs having phosphorylated cyclohexane at the 5' and/or 3'-terminal adenine. These 2'-O-methyl-RNAs formed less stable duplex with the longer target RNA than that with the short target RNA. We tried to improve the short-RNA selective hybridization property by the synthesis of 2'-O-methyl-RNAs having terminal guanine modifications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cyclohexanes / chemistry
  • Nucleic Acid Denaturation
  • Nucleic Acid Hybridization
  • Oligonucleotides / chemical synthesis
  • Oligonucleotides / chemistry
  • Phosphorylation
  • RNA, Untranslated / chemistry*

Substances

  • Cyclohexanes
  • Oligonucleotides
  • RNA, Untranslated
  • Cyclohexane