WNT5A gene is aberrantly methylated from the early stages of colorectal cancers

Hepatogastroenterology. 2009 Jul-Aug;56(93):1007-9.

Abstract

Background/aims: Recently, it has been reported that WNT5A methylation was frequently detected in colorectal cancers. However, the relationship between the WNT5A methylation and the characteristics of these cancers remains unknown.

Methodology: Methylation status of the WNT5A gene was examined in primary carcinomas and the corresponding normal tissues derived from 49 patients with colorectal cancer using quantitative methylation-specific PCR (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated.

Results: Aberrant methylation of the WNT5A gene was detected in 17 out of the 49 (35%) primary colorectal cancers. The clinicopathological data were correlated with the methylation results. All stages of colorectal cancers presented WNT5A methylation.

Conclusions: WNT5A gene has been methylated from the early stages of colorectal cancers.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Chi-Square Distribution
  • Colorectal Neoplasms / genetics*
  • Colorectal Neoplasms / metabolism
  • Colorectal Neoplasms / pathology
  • DNA Methylation*
  • Female
  • Humans
  • Male
  • Middle Aged
  • Neoplasm Staging
  • Polymerase Chain Reaction / methods
  • Proto-Oncogene Proteins / genetics*
  • Wnt Proteins / genetics*
  • Wnt-5a Protein

Substances

  • Proto-Oncogene Proteins
  • WNT5A protein, human
  • Wnt Proteins
  • Wnt-5a Protein