Abstract
Escherichia coli K-12 strains producing S-fimbrial adhesins, F1C fimbriae, and mutagenized fimbriae were tested in a binding assay with a renal tubular cell line. S-fimbrial adhesins and F1C fimbriae mediated binding to tubular cells. The SfaA, SfaG, and SfaS subunits of S fimbriae contributed to attachment. Site-specific mutations in the sfaS gene reduced binding. The inhibition profile of F1C fimbriae resembled that of S fimbriae.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adhesins, Escherichia coli
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Animals
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Bacterial Adhesion*
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Bacterial Outer Membrane Proteins / genetics
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Cell Line
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Escherichia coli / genetics
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Escherichia coli / physiology*
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Fimbriae, Bacterial / immunology
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Fimbriae, Bacterial / physiology*
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Genes, Bacterial
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Kidney Tubules / microbiology*
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Recombinant Fusion Proteins
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Swine
Substances
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Adhesins, Escherichia coli
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Bacterial Outer Membrane Proteins
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Recombinant Fusion Proteins