Abstract
The cDNA for TTF-1, a thyroid nuclear factor that binds to the promoter of thyroid specific genes, has been cloned. The protein encoded by the cDNA shows binding properties indistinguishable from those of TTF-1 present in nuclear extracts of differentiated rat thyroid cells. The DNA binding domain of TTF-1 is a novel mammalian homeodomain that shows considerable sequence homology to the Drosophila NK-2 homeodomain. TTF-1 mRNA and corresponding binding activity are detected in thyroid and lung. The chromosomal localization of the TTF-1 gene has been determined in humans and mice and corresponds to chromosomes 14 and 12, respectively, demonstrating that the TTF-1 gene is not located within previously described clusters of homeobox-containing genes.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites
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Chromosome Mapping
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Chromosomes, Human, Pair 14
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Cloning, Molecular
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DNA / genetics
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DNA-Binding Proteins / genetics*
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Gene Expression
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Genes, Homeobox
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Humans
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Lung / physiology
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Mice
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Molecular Sequence Data
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Nuclear Proteins / genetics*
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Nuclear Proteins / metabolism
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RNA, Messenger / genetics
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Rats
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Regulatory Sequences, Nucleic Acid
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Thyroid Gland / physiology*
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Thyroid Nuclear Factor 1
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Tissue Distribution
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Transcription Factors*
Substances
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DNA-Binding Proteins
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NKX2-1 protein, human
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Nkx2-1 protein, mouse
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Nkx2-1 protein, rat
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Nuclear Proteins
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RNA, Messenger
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Thyroid Nuclear Factor 1
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Transcription Factors
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DNA