The APOA5-1131 T>C variant enhances the association between RBP4 and hypertriglyceridemia in diabetes

Nutr Metab Cardiovasc Dis. 2010 May;20(4):243-8. doi: 10.1016/j.numecd.2009.04.003. Epub 2009 Sep 17.

Abstract

Background and aim: Type 2 diabetic patients have an increased prevalence of hypertriglyceridemia. RBP4 has been associated with insulin resistance and hypertriglyceridemia in obesity, the metabolic syndrome and type 2 diabetes. APOA5 is proposed to be a genetic modulator of triglycerides. The aim of this study was to evaluate the relationship between RBP4 plasma levels and lipid disturbances and to determine the impact of the APOA5-1131 T>C variant on this relationship in type 2 diabetic patients.

Methods and results: A total of 165 type 2 diabetic patients were included in the study. RBP4 plasma levels and the APOA5-1131 T>C variant were determined and the complete lipid profile was assessed by sequential ultracentrifugation. RBP4 was positively correlated with triglyceride levels in plasma and with all the components of triglyceride-rich lipoproteins. Despite the fact that a statistically significant relationship between the APOA5 genetic variant and RBP4 plasma levels was not found, the hypertriglyceridemic effect of high RBP4 levels was enhanced by the presence of the APOA5-1131 T>C genetic variant. Correlation coefficients were 2-fold higher for TC carriers compared to TT carriers with regard to RBP4 plasma levels and all the components of triglyceride-rich lipoproteins. Those type 2 diabetic patients with high RBP4 plasma concentrations and who were TC carriers showed an increased incidence of hypertriglyceridemia (OR=7.46, P=0.010).

Conclusion: RBP4 is associated with hypertriglyceridemia in type 2 diabetic patients. The RBP4 effect is conditioned by the presence of the APOA5-1131 T>C genetic variant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Apolipoprotein A-V
  • Apolipoproteins A / genetics*
  • Apolipoproteins A / physiology
  • Cholesterol, HDL / blood
  • DNA / genetics
  • Diabetes Mellitus, Type 2 / blood
  • Diabetes Mellitus, Type 2 / complications*
  • Diabetes Mellitus, Type 2 / genetics*
  • Female
  • Genetic Variation
  • Genotype
  • Humans
  • Hypertriglyceridemia / etiology*
  • Hypertriglyceridemia / genetics*
  • Lipids / blood
  • Lipoproteins / blood
  • Male
  • Middle Aged
  • Odds Ratio
  • Retinol-Binding Proteins, Plasma / genetics*
  • Retinol-Binding Proteins, Plasma / physiology
  • Triglycerides / blood

Substances

  • APOA5 protein, human
  • Apolipoprotein A-V
  • Apolipoproteins A
  • Cholesterol, HDL
  • Lipids
  • Lipoproteins
  • RBP4 protein, human
  • Retinol-Binding Proteins, Plasma
  • Triglycerides
  • DNA