Abstract
In vivo expression of CO dehydrogenase/acetyl coenzyme A synthase in Methanosarcina spp. is coordinately regulated in response to substrate by at least two mechanisms: differential transcription initiation and early elongation termination near the 3' end of a 371-bp leader sequence. This is the first report of regulation of transcription elongation in the Archaea.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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5' Untranslated Regions / genetics
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5' Untranslated Regions / physiology
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Aldehyde Oxidoreductases / genetics
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Aldehyde Oxidoreductases / physiology*
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Archaeal Proteins / genetics
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Archaeal Proteins / physiology
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Base Sequence
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Gene Expression Regulation, Archaeal / genetics
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Methanosarcina / enzymology*
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Methanosarcina / genetics*
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Molecular Sequence Data
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Multienzyme Complexes / genetics
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Multienzyme Complexes / physiology*
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Homology, Nucleic Acid
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Transcription, Genetic / genetics
Substances
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5' Untranslated Regions
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Archaeal Proteins
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Multienzyme Complexes
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Aldehyde Oxidoreductases
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carbon monoxide dehydrogenase