Chrysanthemum morifolium Ramat (CM) extract protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity

J Ethnopharmacol. 2009 Dec 10;126(3):447-54. doi: 10.1016/j.jep.2009.09.017. Epub 2009 Sep 19.

Abstract

Ethnopharmacological relevance: Chrysanthemum morifolium Ramat (Asteraceae) has (CM) long been used in Korean and Chinese traditional herbal medicines with numerous therapeutic applications.

Aim of the study: To evaluate the neuroprotective activities of Chrysanthemum morifolium (CM) extract against 1-methyl-4-phenylpridinium ions (MPP(+)), Parkinsonian toxin through oxidative stress and impaired energy metabolism, in human SH-SY5Y neuroblastoma cells and the underlying mechanisms.

Materials and methods: The effects of CM against MPP(+)-induced cytotoxicity and neuronal cell viability, oxidative damage, the expression of Bcl-2 and Bax, caspase-3 and poly(ADP-ribose) polymerase (PARP) proteolysis were evaluated by using SH-SY5Y neuroblastoma cells.

Results: CM effectively inhibited the cytotoxicity and improved cell viability. CM also attenuated the elevation of reactive oxygen species (ROS) level, increase in Bax/Bcl-2 ratio, cleavage of caspase-3 and PARP proteolysis.

Conclusion: These results demonstrate that CM possesses potent neuroprotective activity and therefore, might be a potential candidate in neurodegenerative diseases such as Parkinson's disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Methyl-4-phenylpyridinium / toxicity*
  • Base Sequence
  • Caspase 3 / metabolism
  • Cell Line, Tumor
  • Chrysanthemum / chemistry*
  • DNA Primers
  • Humans
  • Neuroblastoma / enzymology
  • Neuroblastoma / metabolism
  • Neuroblastoma / pathology*
  • Neuroprotective Agents / pharmacology*
  • Oxidative Stress
  • Plant Extracts / pharmacology*
  • Poly(ADP-ribose) Polymerases / metabolism
  • Reactive Oxygen Species / metabolism
  • bcl-2-Associated X Protein / metabolism

Substances

  • DNA Primers
  • Neuroprotective Agents
  • Plant Extracts
  • Reactive Oxygen Species
  • bcl-2-Associated X Protein
  • Poly(ADP-ribose) Polymerases
  • Caspase 3
  • 1-Methyl-4-phenylpyridinium