Abstract
Objectives:
To perform genetic analysis of influenza A and B viruses in Myanmar from 2005 to 2007 and to determine the prevalence of amantadine-resistant influenza A viruses.
Methods:
Phylogenies of the HA and NA genes were analyzed and mutations in M2 that confer resistance to amantadine were screened.
Results:
Influenza in Myanmar exhibited seasonality, which coincided during the rainy season from June to August. Out of 2,618 samples, 76 influenza A and 132 influenza B viruses were isolated. Phylogenetic analysis showed that in 2005, 11 A/H1N1 isolates formed one cluster with A/Solomon Islands/3/2006 and were amantadine-sensitive strains. One A/H3N2 isolate was amantadine-resistant harboring S31N mutation in M2 and possessing S193F and D225N substitutions in HA (clade N), similar to A/Wisconsin/67/2005. No viruses were isolated in 2006 due to sample storage failure. In 2007, all 64 A/H3N2 isolates were amantadine-resistant and similar to A/Brisbane/10/2007. For influenza B, 3 Yamagata-lineage and 17 Victoria-lineage isolates were detected in 2005 and 112 Victoria-lineage viruses were isolated in 2007. All Victoria-lineage isolates were reassortants possessing NA derived from the Yamagata lineage.
Conclusion:
Continuous surveillance in tropical countries is important for elucidating the seasonality of influenza and determining the molecular characteristics of circulating strains.
Copyright 2009 S. Karger AG, Basel.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amantadine / pharmacology
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Antiviral Agents / pharmacology
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Drug Resistance, Viral / genetics
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Hemagglutinin Glycoproteins, Influenza Virus / genetics
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Humans
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Influenza A Virus, H1N1 Subtype / drug effects
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Influenza A Virus, H1N1 Subtype / genetics*
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Influenza A Virus, H3N2 Subtype / drug effects
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Influenza A Virus, H3N2 Subtype / genetics*
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Influenza B virus / drug effects
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Influenza B virus / genetics*
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Influenza, Human / drug therapy
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Influenza, Human / epidemiology*
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Influenza, Human / virology
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Molecular Sequence Data
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Myanmar / epidemiology
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Neuraminidase / genetics
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Phylogeny
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RNA, Viral / analysis
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RNA, Viral / genetics
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Seasons
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Sequence Analysis, RNA
Substances
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Antiviral Agents
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Hemagglutinin Glycoproteins, Influenza Virus
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RNA, Viral
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Amantadine
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Neuraminidase
Associated data
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GENBANK/FJ229839
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GENBANK/FJ229840
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GENBANK/FJ229841
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GENBANK/FJ229842
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