Saturation transfer difference NMR reveals functionally essential kinetic differences for a sugar-binding repressor protein

Chem Commun (Camb). 2009 Oct 21:(39):5862-4. doi: 10.1039/b913489a. Epub 2009 Aug 19.

Abstract

The binding kinetics of disaccharides trehalose and trehalose-6-phosphate to repressor protein TreR have been determined using STD NMR and shed light on the contrasting biological roles of these two sugars.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Computer Simulation
  • Escherichia coli Proteins / chemistry*
  • Escherichia coli Proteins / metabolism
  • Kinetics
  • Magnetic Resonance Spectroscopy*
  • Protein Binding
  • Repressor Proteins / chemistry*
  • Repressor Proteins / metabolism
  • Sugar Phosphates / chemistry*
  • Trehalose / analogs & derivatives*
  • Trehalose / chemistry*

Substances

  • Escherichia coli Proteins
  • Repressor Proteins
  • Sugar Phosphates
  • TreR protein, E coli
  • trehalose-6-phosphate
  • Trehalose