Sox-2 in taste bud and lateral line system of zebrafish during development

Neurosci Lett. 2009 Dec 18;467(1):36-9. doi: 10.1016/j.neulet.2009.09.056. Epub 2009 Oct 1.

Abstract

The Sox-2 is a transcription factor involved in adult neurogenesis in different vertebrate species, including fishes. Sox-2 also participates in growth and renewal on sensory cells in neuromasts of the fish lateral line system, and it is essential for development of taste buds in mammals. Using immunohistochemistry and Western blot we have investigated the occurrence and localization of Sox-2 taste buds and neuromast of zebrafish from 10 days post-fertilization to adult stage (1 year). The antibody used identifies two protein bands with estimated molecular weights of 34 and 37kDa which are consistent with those predicted for Sox-2. Sensory cells in taste buds displayed Sox-2 immunoreactivity at all the ages sampled, whereas in the neuromasts Sox-2 expression was restricted to the basal non-sensory cells. Interestingly Sox-2 immunoreactivity was observed in epithelial cells associated with both taste buds and neuromasts. Present results demonstrate that Sox-2 expressed in taste buds and neuromasts of zebrafish during the whole lifespan. Nevertheless, whereas the role of Sox-2 in taste buds of zebrafish remains to be established, the results in neuromast suggest that Sox-2 could participate in cell renewal of the mechanosensory cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Chemoreceptor Cells / metabolism
  • Epithelial Cells / metabolism
  • Female
  • Immunohistochemistry
  • Lateral Line System / embryology
  • Lateral Line System / growth & development*
  • Lateral Line System / metabolism*
  • Male
  • Mechanoreceptors / metabolism
  • SOX Transcription Factors / metabolism*
  • Taste Buds / embryology
  • Taste Buds / growth & development*
  • Taste Buds / metabolism*
  • Zebrafish
  • Zebrafish Proteins / metabolism*

Substances

  • SOX Transcription Factors
  • Sox2 protein, zebrafish
  • Zebrafish Proteins