Abstract
The enteropathogenic Escherichia coli adherence factor (EAF) probe detects isolates of enteropathogenic E. coli that exhibit localized adherence to HEp-2 cells. A 21-base oligonucleotide probe was constructed on the basis of a sequence from within the 1-kb EAF probe and was shown to have greater sensitivity and specificity than the EAF fragment probe in detecting localized adherent E. coli.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adhesins, Escherichia coli
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Bacterial Adhesion
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Bacterial Outer Membrane Proteins / genetics*
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Bacterial Outer Membrane Proteins / isolation & purification
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Base Sequence
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DNA Probes*
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DNA, Bacterial / genetics
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Escherichia coli / genetics*
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Escherichia coli / pathogenicity
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Humans
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Molecular Sequence Data
Substances
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Adhesins, Escherichia coli
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Bacterial Outer Membrane Proteins
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DNA Probes
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DNA, Bacterial