Photodynamic diagnosis (5-aminolevulinic acid) of transitional cell carcinoma after bacillus Calmette-Guérin immunotherapy and mitomycin C intravesical therapy

Eur Urol. 2010 Apr;57(4):655-60. doi: 10.1016/j.eururo.2009.09.037. Epub 2009 Oct 6.

Abstract

Background: Photodynamic diagnosis (PDD) is a technique that enhances the detection of occult bladder tumors during cystoscopy using a fluorescent dye.

Objective: To study the differential effects of bacillus Calmette-Guérin (BCG) and mitomycin C (MMC) intravesical therapy on the false-positive rate of PDD of bladder cancer.

Design, setting, and participants: This study included 552 procedures and 1874 biopsies.

Intervention: Tumors were resected and biopsies were taken from suspicious areas, under guidance of white-light endoscopy and 5-ALA (5-aminolevulinic acid)-induced fluorescence cystoscopy.

Measurements: The influence of intravesical BCG immunotherapy and intravesical MMC chemotherapy on pyuria, inflammation, and PDD specificity was examined in univariate analyses.

Results and limitations: BCG significantly results in inflammation (odds ratio [OR]: 1.53, p=0.002), leukocyturia (OR: 1.84, p=0.034), and false positives in PDD (OR: 1.49, p=0.001). However, a single BCG instillation within 3 mo before PDD is most likely not associated with increased false-positive rates (OR: 0.35, p=0.26). Leukocyturia normalizes within 6 wk after the last BCG instillation, but PDD specificity is reduced up to 3 mo.

Conclusions: BCG is an important predictor for false positives in PDD (5-ALA). More than one BCG instillation within 3 mo before fluorescence cystoscopy decreases the specificity of PDD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Intravesical
  • Aminolevulinic Acid*
  • Antibiotics, Antineoplastic / administration & dosage*
  • Antibiotics, Antineoplastic / adverse effects
  • BCG Vaccine / administration & dosage*
  • BCG Vaccine / adverse effects
  • Biopsy
  • Carcinoma, Transitional Cell / diagnosis*
  • Carcinoma, Transitional Cell / pathology
  • Carcinoma, Transitional Cell / therapy*
  • Cystoscopy*
  • False Positive Reactions
  • Female
  • Humans
  • Inflammation / chemically induced
  • Leukocyte Count
  • Logistic Models
  • Male
  • Mitomycin / administration & dosage*
  • Mitomycin / adverse effects
  • Neoplasm Staging
  • Netherlands
  • Odds Ratio
  • Predictive Value of Tests
  • Pyuria / chemically induced
  • Retrospective Studies
  • Risk Assessment
  • Risk Factors
  • Sensitivity and Specificity
  • Time Factors
  • Treatment Outcome
  • Urinary Bladder Neoplasms / diagnosis*
  • Urinary Bladder Neoplasms / pathology
  • Urinary Bladder Neoplasms / therapy*
  • Urine / cytology

Substances

  • Antibiotics, Antineoplastic
  • BCG Vaccine
  • Mitomycin
  • Aminolevulinic Acid