Abstract
The feature of osteoconductivity, and expression of inductive BMP and transcription factors (Runx2 and Osterix) for osteoblast differentiation, which was related to conductive bone formation, were observed in experimentally created defects in rat femoral and parietal bones filled with beta-tricalcium phosphate (beta-TCP) or carbonate apatite (CAP). Femoral cortical bone defects were repaired by conductive bone formed by osteoblasts differentiated around beta-TCP and CAP, and immunohistochemical observation revealed that the osteoblasts expressed BMPs, Runx2, and Osterix. However, the repair in parietal bone defects was incomplete despite the beta-TCP and CAP filling. Only cells, which differentiated around beta-TCP or CAP, and formed conductive bone expressed BMPs, Runx2, and Osterix. These findings revealed that the osteoconductivity of calcium phosphate materials required the expression of BMPs as the prerequisite for Runx2 and Osterix expression. Therefore, it is suggested that when calcium phosphate ceramics are used as bone substitute materials, BMPs are essential for osteoconductivity.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apatites / pharmacology*
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Biocompatible Materials / pharmacology
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Bone Morphogenetic Proteins / drug effects
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Bone Morphogenetic Proteins / metabolism
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Bone Regeneration / drug effects*
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Bone Regeneration / physiology
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Bone Substitutes / pharmacology*
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Calcium Phosphates / pharmacology*
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Cell Differentiation / drug effects
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Core Binding Factor Alpha 1 Subunit / drug effects
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Core Binding Factor Alpha 1 Subunit / metabolism
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Femur / drug effects
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Femur / metabolism
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Femur / surgery
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Immunohistochemistry
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Longitudinal Studies
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Osteoblasts / drug effects
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Osteoblasts / metabolism
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Osteogenesis / drug effects*
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Osteogenesis / physiology
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Parietal Bone / drug effects
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Parietal Bone / metabolism
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Parietal Bone / surgery
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Rats
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Rats, Wistar
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Time Factors
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Transcription Factors / drug effects
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Transcription Factors / metabolism
Substances
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Apatites
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Biocompatible Materials
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Bone Morphogenetic Proteins
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Bone Substitutes
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Calcium Phosphates
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Core Binding Factor Alpha 1 Subunit
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Runx2 protein, rat
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Sp7 protein, rat
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Transcription Factors
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beta-tricalcium phosphate
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carboapatite