Abstract
Targeted RNA-Seq combines next-generation sequencing with capture of sequences from a relevant subset of a transcriptome. When testing by capturing sequences from a tumor cDNA library by hybridization to oligonucleotide probes specific for 467 cancer-related genes, this method showed high selectivity, improved mutation detection enabling discovery of novel chimeric transcripts, and provided RNA expression data. Thus, targeted RNA-Seq produces an enhanced view of the molecular state of a set of "high interest" genes.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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DNA, Complementary / genetics
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Gene Expression Profiling / methods*
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Gene Expression Regulation, Neoplastic / genetics
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Humans
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K562 Cells
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Molecular Sequence Data
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Mutation / genetics*
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Neoplasms / genetics*
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Oncogene Proteins, Fusion / chemistry
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Oncogene Proteins, Fusion / genetics*
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Oncogene Proteins, Fusion / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Sequence Analysis, DNA / methods*
Substances
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DNA, Complementary
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Oncogene Proteins, Fusion
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RNA, Messenger