Ferulic acid induces neural progenitor cell proliferation in vitro and in vivo

Neuroscience. 2010 Jan 20;165(2):515-24. doi: 10.1016/j.neuroscience.2009.10.023.

Abstract

Ferulic acid (4-hydroxy-3-methoxycinnamic acid; FA) is a plant constituent and is contained in several medicinal plants for clinical use. In this paper, we investigated the effects of FA on the proliferation of neural stem/progenitor cells (NSC/NPCs) in vitro and in vivo. FA significantly increased the proliferation of NSC/NPCs cultured from the telencephalon of embryonic day-14 rats, and increased the number and size of secondary formed neurospheres. An in vitro differentiation assay showed that FA did not affect the percentage of either neuron-specific class III beta-tubulin (Tuj-1)-positive cells or glial fibrillary acidic protein (GFAP)-positive cells in the total cell population. Oral administration of FA increased the number of newly generated cells in the dentate gyrus (DG) of the hippocampus of corticosterone (CORT)-treated mice, indicating that FA enhances the proliferation of adult NSC/NPCs in vivo. We also found that oral administration of FA increased cAMP response element binding protein (CREB) phosphorylation and brain-derived neurotrophic factor (BDNF) mRNA level in the hippocampus of CORT-treated mice, and ameliorated the stress-induced depression-like behavior of mice. These novel pharmacological effects of FA may be useful for the treatment of mood disorders such as depression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging
  • Animals
  • Cell Differentiation / drug effects
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Central Nervous System Agents / pharmacology*
  • Corticosterone / pharmacology
  • Coumaric Acids / pharmacology*
  • Dentate Gyrus / drug effects
  • Dentate Gyrus / physiology
  • Depression / drug therapy
  • Depression / etiology
  • Hippocampus / drug effects
  • Hippocampus / physiology
  • Male
  • Mice
  • Neurogenesis / drug effects*
  • Neurons / drug effects*
  • Neurons / physiology
  • Rats
  • Rats, Wistar
  • Stem Cells / drug effects*
  • Stem Cells / physiology
  • Stress, Psychological / complications
  • Stress, Psychological / drug therapy
  • Telencephalon / drug effects
  • Telencephalon / physiology

Substances

  • Central Nervous System Agents
  • Coumaric Acids
  • ferulic acid
  • Corticosterone