Monocyte-derived interleukin-10 depresses the Bordetella pertussis- specific gamma interferon response in vaccinated infants

Clin Vaccine Immunol. 2009 Dec;16(12):1816-21. doi: 10.1128/CVI.00314-09. Epub 2009 Oct 21.

Abstract

Antigen-specific gamma interferon (IFN-gamma) has been demonstrated to participate in protection against Bordetella pertussis infection. Circulating mononuclear cells from B. pertussis-infected and from pertussis-vaccinated infants secrete high amounts of IFN-gamma after in vitro stimulation by B. pertussis antigens, but with a large variation in the secreted IFN-gamma levels between individuals. We show here that the inhibition of the specific IFN-gamma response can be at least partially attributed to IL-10 secretion by monocytes. This IL-10 secretion was not associated with polymorphisms at positions -1082, -819, and -592 of the IL-10 gene promoter, suggesting that other genetic or environmental factors affect IL-10 expression and secretion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesins, Bacterial / pharmacology
  • Alleles
  • Antibodies, Monoclonal / pharmacology
  • Bordetella pertussis / immunology*
  • Genotype
  • Humans
  • Immunologic Factors / pharmacology
  • Infant
  • Interferon-gamma / agonists
  • Interferon-gamma / biosynthesis
  • Interferon-gamma / immunology
  • Interleukin-10 / antagonists & inhibitors
  • Interleukin-10 / biosynthesis
  • Interleukin-10 / genetics
  • Interleukin-10 / immunology*
  • Interleukin-12 / agonists
  • Interleukin-12 / biosynthesis
  • Interleukin-12 / immunology
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / immunology*
  • Leukocytes, Mononuclear / metabolism
  • Leukocytes, Mononuclear / microbiology
  • Longitudinal Studies
  • Pertussis Toxin / pharmacology
  • Pertussis Vaccine / immunology*
  • Polymorphism, Genetic / genetics
  • Polymorphism, Genetic / immunology
  • Virulence Factors, Bordetella / pharmacology
  • Whooping Cough / immunology*
  • Whooping Cough / microbiology
  • Whooping Cough / prevention & control

Substances

  • Adhesins, Bacterial
  • Antibodies, Monoclonal
  • Immunologic Factors
  • Pertussis Vaccine
  • Virulence Factors, Bordetella
  • filamentous hemagglutinin adhesin, Bordetella pertussis
  • Interleukin-10
  • Interleukin-12
  • Interferon-gamma
  • Pertussis Toxin