Chronic acetonemia alters liver oxidative balance and lipid content in rats. A model of NASH?

Exp Clin Endocrinol Diabetes. 2010 Jan;118(1):61-3. doi: 10.1055/s-0029-1225649. Epub 2009 Oct 23.

Abstract

Acetone is considered to be a substance that can disturb cellular oxidative status, being also associated with the production of glucose during its metabolization. The objective of the present study was to determine the effects of chronic treatment with acetone in oxidative stress and metabolic parameters in rats. Twenty male Wistar rats were divided into two groups: control (CG) and chronic acetone group (CAG). After 28 days of acetone ingestion in a 5% aqueous solution (CAG) or water (CG) the animals were euthanized and urine, plasma and liver were collected for the determination of acetone, glucose, lipemia, hepatic fat, malondialdehyde (MDA), reduced glutathione (GSH), and vitamin E. As expected, urinary and plasma acetone levels were higher in CAG. There was no difference in hepatic MDA values between groups, whereas hepatic GSH was lower in CAG than in CG and hepatic vitamin E was higher in CAG than in CG. There was also an increase in glycemia, cholesterolemia and hepatic fat in CAG compared to CG. Chronic treatment with a 5% acetone solution produced an increase in acetonemia that was able to promote changes in hepatic oxidative metabolism and in lipid content in rats similar to those observed in nonalcoholic steatohepatitis.

MeSH terms

  • Acetone / toxicity*
  • Animals
  • Blood Glucose / analysis
  • Cholesterol / blood
  • Chronic Disease
  • Disease Models, Animal
  • Fatty Liver / chemically induced
  • Fatty Liver / metabolism*
  • Glutathione / metabolism
  • Ketone Bodies / blood
  • Ketone Bodies / urine
  • Ketosis / metabolism*
  • Lipid Metabolism*
  • Liver / drug effects
  • Liver / metabolism*
  • Male
  • Oxidative Stress*
  • Rats
  • Rats, Wistar
  • Solvents / toxicity*
  • Thiobarbituric Acid Reactive Substances / metabolism
  • Triglycerides / blood
  • Vitamin E / blood
  • Vitamin E / metabolism

Substances

  • Blood Glucose
  • Ketone Bodies
  • Solvents
  • Thiobarbituric Acid Reactive Substances
  • Triglycerides
  • Acetone
  • Vitamin E
  • Cholesterol
  • Glutathione