Abstract
The Xenopus laevis TFIIIA promoter contains a motif that has been implicated in promoter activation in late-stage oocytes and contains the sequence (-269) CACGTG (-264). A cDNA encoding a protein (B1) that binds to this element has been cloned from X. laevis and Xenopus borealis ovarian cDNA libraries. We show that this protein is a member of the helix-loop-helix family of regulatory proteins and contains 80% sequence identity with the human adenovirus major late transcription factor (MLTF or USF). A survey of B1 protein expression during oogenesis and embryogenesis revealed both oocyte-specific and somatic cell-specific B1 protein-DNA complexes. Immunological data, RNA blot analysis, and proteolytic clipping band shift assays indicated that these complexes most likely represent altered forms of a single B1 polypeptide. Implications for TFIIIA gene regulation during development are discussed.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
Blotting, Northern
-
Cloning, Molecular
-
DNA / genetics
-
DNA-Binding Proteins / genetics
-
Gene Expression Regulation*
-
Genes
-
Molecular Sequence Data
-
Oligonucleotides / chemistry
-
Oocytes / physiology
-
Polymerase Chain Reaction
-
Promoter Regions, Genetic*
-
Protein Processing, Post-Translational
-
RNA, Messenger / genetics
-
Regulatory Sequences, Nucleic Acid
-
Transcription Factor TFIIIA
-
Transcription Factors / genetics*
-
Transcription Factors / physiology*
-
Upstream Stimulatory Factors
-
Xenopus / genetics
-
Xenopus laevis / embryology
-
Xenopus laevis / physiology*
Substances
-
B1 factor, Xenopus
-
DNA-Binding Proteins
-
Oligonucleotides
-
RNA, Messenger
-
Transcription Factor TFIIIA
-
Transcription Factors
-
Upstream Stimulatory Factors
-
DNA
Associated data
-
GENBANK/D00651
-
GENBANK/D00652
-
GENBANK/D00653
-
GENBANK/D00654
-
GENBANK/M63663
-
GENBANK/M63664
-
GENBANK/M64825
-
GENBANK/M64826
-
GENBANK/M64827
-
GENBANK/S43885