A micropatterned cell array with an integrated oxygen-sensitive fluorescent membrane

Photochem Photobiol Sci. 2009 Nov;8(11):1529-33. doi: 10.1039/b9pp00061e. Epub 2009 Sep 30.

Abstract

We propose a simple method for producing micropatterned cell spots by photocatalytic lithography on a Pt porphyrin-based oxygen-sensitive polystyrene membrane that enables real-time imaging of oxygen consumption of patterned cell spots with sub-millimetre resolution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Respiration / drug effects
  • Dose-Response Relationship, Drug
  • Hep G2 Cells
  • Humans
  • Membranes, Artificial*
  • Microscopy, Fluorescence
  • Oxygen / metabolism*
  • Polystyrenes / chemistry
  • Porphyrins / chemistry
  • Sodium Cyanide / pharmacology
  • Tissue Array Analysis / methods*

Substances

  • Membranes, Artificial
  • Polystyrenes
  • Porphyrins
  • Sodium Cyanide
  • Oxygen