Abstract
A multiplex PCR-ligation detection reaction (PCR-LDR) assay was developed for rapid detection of methicillin, tetracycline, and vancomycin resistance, as well as toxic shock toxin and Panton-Valentine leukocidin. The assay was tested on 470 positive blood culture bottles containing Staphylococcus aureus or enterococci. PCR-LDR exhibited a sensitivity and specificity of > or = 98% for all components except tetracycline resistance, which had a sensitivity of 94.7%. Rapid and sensitive detection of antimicrobial resistance and virulence genes could help guide therapy and appropriate infection control measures.
Publication types
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Evaluation Study
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Research Support, N.I.H., Extramural
MeSH terms
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Bacterial Toxins / genetics*
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Bacteriological Techniques / methods*
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Blood / microbiology
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DNA, Bacterial / genetics
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Drug Resistance, Bacterial*
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Enterococcus faecalis / genetics*
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Enterococcus faecalis / isolation & purification
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Enterococcus faecium / genetics*
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Enterococcus faecium / isolation & purification
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Gram-Positive Bacterial Infections / microbiology
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Humans
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Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Staphylococcus aureus / genetics*
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Staphylococcus aureus / isolation & purification
Substances
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Bacterial Toxins
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DNA, Bacterial