Objective: To explore TRPV1 UTR-3 polymorphism and susceptibility of childhood asthma of Han Nationality in Beijing.
Methods: 177 asthmatics, 44 atopy, and 151 control children less than 14-years-old were enrolled in case-control study, and all subjects were investigated by ISSAC questionnaires. Dominant, recessive, co-dominant, over-dominant, and log additive model were used to do genotype analysis, and LD analysis and haplotypes of SNPs were tested by Haploview 4.1. Hardy-Weinberg equilibrium test, Person chis-square test, linkage disequilibrium analysis, and logistic analysis were performed by SAS 9.1 software to determine the association between polymorphisms of TRPV1 and susceptibility of childhood asthma.
Results: Polymorphisms were found in rs402369, rs4790521, and rs4790522, Hardy-Weinberg P > 0.05. As to allele frequencies, frequency of SNP rs4790521 T/C in asthmatics were significantly increased (P < 0.05), no significant difference were found in MAF of rs402369 and rs4790522 (P > 0.05). Genotype analysis showed that rs4790521 C/C and rs4790522 A/C were associated with childhood asthma (P < 0.05), and odd ratios were 2.94 (1.32 - 6.53) and 0.588 (0.376 - 0.920) respectively. LD were found between rs4790521 and rs4790522, 3 haplotypes were built. Adjusted by age, gender, parent asthma history, and smoking exposure, logistic stepwise analysis showed that MAF of rs4790521, allozygote C/C of rs4790521, and haplotype C/C were associated with susceptibility to childhood asthma in Chinese Han Nationality in Beijing (P < 0.05).
Conclusion: TRPV1 UTR-3 polymorphisms could be associated with the susceptibility to childhood asthma of Han Nation a city in Beijing.