Distribution of circulating cells in systemic juvenile idiopathic arthritis across disease activity states

Clin Immunol. 2010 Feb;134(2):206-16. doi: 10.1016/j.clim.2009.09.010. Epub 2009 Oct 29.

Abstract

Juvenile idiopathic arthritis (JIA) encompasses a group of chronic childhood arthritides of unknown etiology. One subtype, systemic JIA (SJIA), is characterized by a combination of arthritis and systemic inflammation. Its systemic nature suggests that clues to SJIA pathogenesis may be found in examination of peripheral blood cells. To determine the immunophenotypic profiles of circulating mononuclear cells in SJIA patients with different degrees of disease activity, we studied PBMC from 31 SJIA patients, 20 polyarticular JIA patients (similar to adult rheumatoid arthritis), and 31 age-matched controls. During SJIA disease flare, blood monocyte numbers were increased, whereas levels of myeloid dendritic cells (DC) and gammadelta T cells were reduced. At both flare and quiescence, increased levels of CD14 and CD16 were found on SJIA monocytes. Levels of CD16-DC were elevated at SJIA quiescence compared both to healthy controls and to SJIA subjects with active disease. Overall, our findings suggest dysregulation of innate immunity in SJIA and raise the possibility that quiescence represents a state of compensated inflammation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Arthritis, Juvenile / blood*
  • Arthritis, Juvenile / immunology*
  • B-Lymphocytes / immunology
  • Cell Separation
  • Child
  • Child, Preschool
  • Dendritic Cells / immunology*
  • Female
  • Flow Cytometry
  • Humans
  • Immunophenotyping
  • Killer Cells, Natural / immunology
  • Leukocytes, Mononuclear / immunology*
  • Male
  • Monocytes / immunology*
  • T-Lymphocyte Subsets / immunology
  • T-Lymphocytes / immunology