Translating metabolic exchange with imaging mass spectrometry

Nat Chem Biol. 2009 Dec;5(12):885-7. doi: 10.1038/nchembio.252. Epub 2009 Nov 8.

Abstract

Metabolic exchange between an organism and the environment, including interactions with neighboring organisms, is important for processes of organismal development. Here we develop and use thin-layer agar natural product MALDI-TOF imaging mass spectrometry of intact bacterial colonies grown on top of the MALDI target plate to study an interaction between two species of bacteria and provide direct evidence that Bacillus subtilis silences the defensive arsenal of Streptomyces coelicolor.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus subtilis / genetics
  • Bacillus subtilis / growth & development
  • Bacillus subtilis / metabolism*
  • Bacterial Proteins / biosynthesis*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Genes, Bacterial
  • Mutation
  • Quorum Sensing* / genetics
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Streptomyces coelicolor / genetics
  • Streptomyces coelicolor / growth & development
  • Streptomyces coelicolor / metabolism*

Substances

  • Bacterial Proteins

Associated data

  • PubChem-Substance/85244562
  • PubChem-Substance/85244563
  • PubChem-Substance/85244564
  • PubChem-Substance/85244565
  • PubChem-Substance/85244566
  • PubChem-Substance/85244567
  • PubChem-Substance/85244568
  • PubChem-Substance/85244569
  • PubChem-Substance/85244570
  • PubChem-Substance/85244571
  • PubChem-Substance/85244572
  • PubChem-Substance/85244573
  • PubChem-Substance/85244574
  • PubChem-Substance/85244575
  • PubChem-Substance/85244576
  • PubChem-Substance/85244577