Molecular activation of NF-kappaB, pro-inflammatory mediators, and signal pathways in gamma-irradiated mice

Biotechnol Lett. 2010 Mar;32(3):373-8. doi: 10.1007/s10529-009-0165-4.

Abstract

The effects of gamma-irradiation on inflammatory gene expression, including NF-kappaB activation, in the kidney of C57/BL6 mice exposed to 1-9 Gy doses of (60)Co gamma-irradiation. Radiation enhanced the NF-kappaB activation and oxidative stress caused a dose-dependent disruption in the redox balance. The significance of this study is the new molecular information gained on gamma-irradiation effects through the activation of pro-inflammatory genes by NF-kappaB via the MAPK signaling pathway. Considering the exquisite sensitivity of NF-kappaB and other pro-inflammatory mediators to the redox status, we conclude that the activation of inflammatory processes by irradiation is likely initiated by increased oxidative stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cobalt Radioisotopes
  • Enzyme Activation / radiation effects
  • Gamma Rays*
  • Glutathione Disulfide / metabolism
  • Inflammation Mediators / metabolism*
  • Kidney / metabolism
  • Kidney / radiation effects
  • Lipid Peroxidation / radiation effects
  • Mice
  • Mice, Inbred C57BL
  • Mitogen-Activated Protein Kinases / metabolism
  • NF-kappa B / metabolism*
  • Oxidation-Reduction / radiation effects
  • Reactive Oxygen Species / metabolism
  • Signal Transduction / radiation effects*
  • Up-Regulation / radiation effects
  • Whole-Body Irradiation / methods*

Substances

  • Cobalt Radioisotopes
  • Inflammation Mediators
  • NF-kappa B
  • Reactive Oxygen Species
  • Mitogen-Activated Protein Kinases
  • Glutathione Disulfide