Abstract
Pyrosequencing was used to rapidly detect aac(6')-Ib and aac(6')-Ib-cr genes. This plasmid-mediated quinolone resistance determinant is increasing in extended-spectrum beta-lactamase-producing Enterobacteriaceae. This method is faster and more cost-effective than the methods previously described. Sequences obtained with this pyrosequencing method showed 100% concordance with conventional sequencing.
Publication types
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Comparative Study
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Evaluation Study
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Bacteriological Techniques / methods
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Base Sequence
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Drug Resistance, Bacterial*
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Enterobacteriaceae / drug effects*
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Enterobacteriaceae / genetics*
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Genes, Bacterial*
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Humans
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Molecular Sequence Data
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Plasmids
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Quinolones / pharmacology*
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Sensitivity and Specificity
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Sequence Analysis / methods*
Substances
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Anti-Bacterial Agents
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DNA, Bacterial
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Quinolones